Preparation and properties of highly purified diaphorase
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چکیده
منابع مشابه
The Preparation and Properties of Highly Purified Ascorbic Acid Oxidase
1. A method is described for the preparation of a highly purified ascorbic acid oxidase containing 0.24 per cent copper. 2. Using comparable activity measurements, this oxidase is about one and a half times as active on a dry weight basis as the hitherto most highly purified preparation described by Lovett-Janison and Nelson. The latter contained 0.15 per cent copper. 3. The oxidase activity is...
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The amino acid and lipide composition of a highly active toxohormone preparation (TH2) from human malignant tissues was investigated. Approximately 80 per cent of the TH: could be accounted for as polypeptide, with leucine, alanine, glutamic, and aspartic acid being present in the highest concentrations. The remaining ~0 per cent consisted largely of phospholipides. The TH~ fraction was divided...
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Procedures are described for the preparation of highly purified streptokinase by column chromatography on diethylaminoethyl cellulose and by column electrophoresis in a sucrose density gradient. Preparations chromatographed at least twice on diethylaminoethyl cellulose are shown to be essentially monodisperse on the basis of ultracentrifugal and gel electrophoretic analyses and constancy of spe...
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آنزیم تریپسین در شرایط قلیایی ناپایدار می باشد .و فعالیت پروتئولیتیکی تریپسین منجربه خود هضمی آن در جایگاههای خاصی می گردد. بنابر این آنزیمی با ناپایداری بالا محسوب میگردد. در سالهای اخیر موفق شدند که با ایجاد تغیرات شیمیایی با اضافه کردن فلزات خاص ، کلسیم و یا عمل استیلاسیون منجر به افزایش پایداری آنزیم تریپسین گردند. مطالعات در حال حاضر نشان می دهد که تریپسین استیله شده فعالیت آنزیمی خود را ...
15 صفحه اولProperties of highly purified fucan.
Fucan was first isolated by Kylin from brown seaweed and named fucoidin by him (1). More recently fucan was isolated by Percival and Ross (2) from several Phaeophyceae. They considered it to be a polymeric salt of fucose monosulfate. However, their most highly purified preparation still contained approximately 5.6% sugars other than L-fucose and 3.3 ‘% uranic acid. A branched structure was sugg...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1957
ISSN: 0306-3283
DOI: 10.1042/bj0670146